Contact an Account ManagerSensitive HPLC method for the determination of labetalol in human plasma using fluorimetric detection
Summary
Type: HPLC
Application #: 104120
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: fluorescence, excitation 335nm, emission 370nm
Conditions: eluent: water - acetonitrile (68:32) containing 40g/l NaClO4, 4g/l Na acetate with or without 40g/l trimethylammonium chloride
flow rate 1ml/min
temperature: ambient
postcolumn addition of borate buffer, flow rate 0.23ml/min, adjusting pH to about 9.3
Authors: Oosterhuis, B
Source: J Chromatogr 226 (1981) 259–265
Details
Legend: Fig. 4. Chromatograms of a standard sample containing 125ng labetalol and 100ng
chloroquine, BI eluent without TMA (pH corrected with HCl), BII eluent with 4%
TMA.